Practice question

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Question

Protein A and protein B form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to 200 kDa. SDS-PAGE analysis of this complex, with and without beta-mercaptoethanol, showed a single band corresponding to molecular weight 50 and 25 kDa, respectively. Given that the molecular weight of protein A is 25 kDa, the molecular weight of protein B is ....... kDa .

Explanation

Calculation based on standard biochemistry principles yields 25 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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