Practice question
Question
One litre of phosphate buffer was prepared by adding 208 grams of Na ₂ HPO ₄ (Mol. wt. 142) and 71 grams of NaH ₂ PO ₄ (Mol. wt. 120) in water. If the pKa for the dissociation of H ₂ PO ₄ ⁻ into HPO ₄ 2 ⁻ andH ⁺ is 6.86, the pH of the buffer will be _____________.
Explanation
Mechanistically, enzyme kinetics involves substrate binding and catalytic efficiency determine reaction rates. This validates Option NAT because it reflects active-site specificity and regulation, a pattern repeatedly demonstrated in biochemistry research.
Related blogs
Guides and insights connected to this topic
Eukaryotic DNA Isolation: Principle, Materials, Procedure and Results
What is DNA Extraction? Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other...
Electrophoretic separation of protein by SDS-PAGE
IntroductionElectrophoresis is the process of migration of charged molecules in response to an electric field. The rate of migration depends...
Preparation of Buffer
IntroductionA buffer solution is one that resist pH change on addition of acid. This solution are required in many biochemical experim...
Discussion
Comments
Please log in to join the discussion.
Login to commentNo comments yet. Be the first to start the discussion.