Practice question
Question
In a confirmatory test for HIV, one or more viral antigens are detected in the blood of patients. Following are the steps to be performed for the experiment: A. Transfer of viral antigens to nitrocellulose paper B. Incubation with the buffer containing antibodies specific for viral antigens. C. Separation of viral antigens by SDS-PAGE D. Detection of bands by enzyme-linked secondary antibody Identify the correct sequence steps to be performed for the experiment.
Explanation
in a confirmatory test for hiv, one operates via C-A-B-D represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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