Practice question

MCQ

Question

Given below are the restriction profiles obtained upon digestion of a 4.8 kb plasmid with four different enzymes (E ₁, E ₂, E ₃ and E ₄₎ . E₁: 4.8 kb E₁+E₂= 500 bp + 4.3 kb E₂: 4.8 kb+ E₂+E₃= 400bp+ 4.4 kb E₃ : 4.8 kb+ E₃ + E₄ = 2 kb+ 2.8 kb E₄: 4.8 kb+ E₁+ E₄= 1.9 kb +2.9 kb Based on the above information, which one of the following statements is correct?

Options

Choose one · Correct answer highlighted

E ₁ and E4 have two recognition sites each in the plasmid. E ₂ recognitioin sequence is located between E1 and E3 . Correct The sequence of recognition sites after digestion of the plasmid with E₃ is: E₄-E₂-E₁. The sequence of recognition sites after digestion of the plasmid with E₂ is E₁-E₃ E₄

Explanation

given below are the restriction profiles obtained operates via E ₂ recognitioin sequence is located between E1 and E3 . represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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