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Practice question

Question

E. coli is inoculated in a shake flask containing nutrient rich medium. The initial number of viable cells in the medium is 102. After few hours, the number of viable cells is 106. Assuming cell divides by binary fission, the number of generations that have taken place is ________ (rounded off to the nearest integer)

Explanation

Calculation based on standard cell principles yields 13 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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