Practice question

Limited nuclease digestion in presence of H1 yields larger protected fragment than core alone. Chromatosome comprises nucleosome core particle of 147 base pairs wrapped around octamer plus additional twenty base pairs of linker DNA, ten on each side of entry exit, sealed by globular winged-helix domain of histone H1 contacts. Total DNA content becomes about 167 base pairs, determined by sequencing H1-containing fragments. Structurally H1 locks DNA crossover angle, directing trajectory of adjacent linkers for higher-order folding and reducing entry-exit breathing. Chromatosome represents complete structural unit of condensed chromatin before 30 nanometer fiber assembly.