Practice question

DIF-1 biosynthetic genes stlB encoding polyketide synthase and dmtA encoding desmethyl DIF-1 methyltransferase are preferentially transcribed in prespore region under cAMP and PKA induction. Prespore cells therefore synthesize chlorinated polyketide and release it, where it diffuses to anterior prestalk population acting paracrinally. Prestalk cells contain receptors and downstream STAT machinery but low biosynthetic capacity, functioning as responders. Stalk cells and mature spores show negligible synthesis. This spatial separation creates feedback loop ensuring prespore majority controls stalk proportion; disruption of prespore-specific DIF production alters ratio, producing fruiting bodies with inadequate stalk support and reduced spore elevation.