Practice question

Question

Based upon phenotypic observation, it was concluded that an unknown gene responsible for an agronomically important trait is present in a particular plant . In order to identify the gene, a researcher proposes to use the following strategies: A. PCR amplification of the gene. B. Map based cloning of the gene. C. Subtractive DNA hybridization D. Genome sequencing E. Develop molecular markers linked to the trait Which one of the following options is most suitable for identifying unknown gene?