Practice question

Affinity of a ligand–receptor interaction is quantified by the dissociation constant Kd. In a Scatchard plot the bound/free ligand ratio is graphed against bound ligand concentration. The resulting straight line has a slope equal to –1/Kd. Therefore the numerical value of the slope directly reports affinity: the steeper the negative slope, the higher the affinity. This linearization remains a classical method for extracting both affinity and receptor density from equilibrium binding data. Careful