Practice question

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Question

A UV-visible spectrophotometer has a minimum detectable absorbance of 0.02. The minimum concentration of a protein sample that can be measured reliably in this instrument with a cuvette of 1 cm path length is 11M. The molar extinction coefficient of the protein is 10,000 L mol^-1 cm^-1.

Explanation

Calculation based on standard biochemistry principles yields 2 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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