Practice question
Question
A protein X is active when phosphorylated on Thr residue. You wish to mimic this phosphorylation by mutating Thr to another residue. Which one of these residues will you mutate Thr into?
Explanation
In biochemistry, enzyme catalysis and lipid structure dictates that substrate affinity, transition state stabilization and phospholipid composition govern function. Therefore Glu is correct as it reflects catalytic efficiency and membrane organization, supported by mechanistic studies and conserved across related systems.
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