Practice question
Question
A protein undergoes post-translational modifications. In an experiment to identify the nature of modifications, the following experimental results were obtained. A. Protein moved more slowly in an SDS PAGE. B. Isoelectric focusing (IEF) showed that there was no change in the pI. C. Mass spectrometric analysis showed that the modification was on serineThe modification that the protein undergoes is likely to be
Explanation
a protein undergoes post-translational modifications. in an operates via glycosylation represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
Discussion
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