Practice question
Question
A microorganism grows in a continuous 'chemostat' culture of 60 m^3 working volume with sucrose as the growth limiting nutrient at dilution rate, D = 0.55 h^- 1 . The steady state biomass concentration is 4.5 Kg dry biomass m^·3 and the residual sucrose concentration is 2.0 Kg m^·3. The sucrose concentration in the incoming feed medium is 10.0 Kg m·^3 What would be the sucrose concentration in the input feed for the output to be 45 Kg biomass h^- 1?
Explanation
In bioprocess, 3.225 Kg m^·3 accurately describes the phenomenon since 3.225 kg m^·3 aligns with observed data. Other options like 4.425 Kg m^·3, 5.115 Kg m^·3 ignore key regulatory steps or propose implausible interactions, failing to satisfy thermodynamic and structural constraints.
Discussion
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