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Practice question

Question

A DNA solution of 50 μg mL⁻¹ concentration gives an absorbance of 1.0 at 260 nm. An aliquot of 20 μL from a 50 μL purified plasmid solution is diluted with distilled water to a total volume of 1000 μL. The diluted plasmid solution gives an absorbance of 0.550 at 260 nm. The concentration of the purified plasmid in μg μL⁻¹ (rounded off to two decimal places) is ___

Explanation

Calculation based on standard molecular principles yields 1.38 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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