Practice question
Question
A batch sterilizer is being operated at 121 ᵒ C for sterilizing a medium containing microbial cells. Assume that the thermal deactivation of cells is a first order process with a death rate constant of 0.69 min ⁻¹ at 121 ᵒ C. If the initial concentration of microbes in the medium is 1010 cells m ⁻³ , the time taken to reduce the microbial load to a final concentration of 10 cells m ⁻³ is _________ min. (rounded off to the nearest integer)
Explanation
Calculation based on standard cell principles yields 30 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
Discussion
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