Practice question

MCQ

Question

Q136 In resting cells, proteins X and Y are localized in the cytosol. Upon stimulation with lipopolysaccharide (LPS), both of them are phosphorylated and translocate to the nucleus. You have used antibodies against phosphorylated forms of proteins X and Y which are conjugated to either red, or green or blue dye. Keeping optical aberration of light in mind, which one of the following will be the best for visualizing X and Y In the nucleus by fluorescence microscopy?

Options

Choose one · Correct answer highlighted

Anti green X and anti red Y Anti red X and anti green Y Anti red X and anti blue Y Correct Anti blue X and anti green Y

Explanation

q136 in resting cells, proteins x and operates via Anti red X and anti blue Y represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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