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Practice question

MCQ

Question

Q141 A gene is regulated by a novel transcription factor. The following techniques may be used to identify the cis- regulatory element in the 1 kb promoter sequence of the gene where the novel transcription factor binds: A. Bioinformatics analysis. B. Cell based reporter assay. C. S 1 nuclease assay D. Electrophoretic mobility shift assay. E. DNAse-I foot-printing analysis Which one of the following can help to identify the cis element?

Options

Choose one · Correct answer highlighted

Explanation

Analysis of q141 a gene is regulated by a shows end-labeled probe generates nested fragments on sequencing PAGE revealing protected gaps. DNase I protection supports option D, whereas distractors confuse random priming and fail to reproduce observed results.

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