Practice question

MCQ

Question

Mouse lgM (whole molecule) was injected into rabbit to generate antiserum. Which one of the following mouse antibody components (the same mouse from which IgM molecules were used for Immunization) has the possibility to be recognized using the rabbit antiserum in Western blotting?

Options

Choose one · Correct answer highlighted

IgG F(ab')₂ fragment only J chain only IgG Fe fragment only Both lgG F(ab')₂ fragment and J chain Correct

Explanation

mouse lgm (whole molecule) was injected into operates via Both lgG F(ab')₂ fragment and J chain represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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