Practice question

Question

Following statements are made regarding the properties of two- photon microscopy aver traditional confocal microscopy: A. By using longer wavelength, two-photon microscopy induces less photobleaching of the tissue preparation. B. By using shorter wavelength, two-photon microscopy Induces less photobleaching of the tissue preparation. c. The intensity of fluorescence emitted by the sample will remain the same even if only one of the two exciting photons Impinge an the sample. D. No fluorescence is detected unless two exciting photons simultaneously impinge an the sample. Which one of the following combination of statements Is correct?