Practice question

Question

The following statements are made regarding developing a transgenic mouse: A. The transgenic mouse thus born will be a homozygous transgenic animal and can be maintained by crossing with another transgenic animal. B. The fertilized transgenic eggs are allowed to develop in vitro. C. The desired gene is preferably microinjected in male pronucleus after sperm entry in oocyte. D. For best efficiency, the desired gene is always microinjected in the male gametes and then they are allowed to fertilize the female gametes. E. Blastocyst stage embryos are transferred to the uterus of hormonally prepared mother. F. The fertilized eggs are collected from specific strain of mouse. G. The female mouse of specific strain is super ovulated, oocytes are collected and allowed to fertilize in vitro. Choose the combination of statements arranged in the correct sequence for developing transgenic mouse.