Practice question
Question
Some of the following transgenic approaches could be used for functional characterization of endogenous genes in plants: A. Transformation using a binary vector containing a strong enhancer element and lacking the right border of T-DNA B. Transformation using a binary vector containing a promoter-less reporter gene sequence and a selction maker gene cassette within the T-DNA. C. Transformation using a binary vector containing only a strong enhancer element and a selection marker gene cassette within the T-DNA. D. Transformation using a binary vector lacking a reporter gene as well as both the left and right borders of T-DNA. Which one of the following combinations can be used?
Explanation
some of the following transgenic approaches could operates via B and C only represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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