Practice question

Question

Given below are four statements on various methods used in molecular biology and recombinant DNA studies: A. Orientation of a cloned DNA fragment in a plasmid vector can be determined by using primers specific to the cloned fragment. B. Transcriptome sequencing cannot be used to identify differential expression profiles of mRNA in an organism. C. A DNA fragment with 5' overhangs can be converted into a blunt-ended fragment by using a nuclease but not by a DNA polymerase D. Oligonucleotide probes can be labelled at their 5 and 3' ends by using T4polynucleotide kinase and terminal transferase, respectively Which one of the following options consists of only correct statement/s?