Practice question

Bidirectional unwinding of parental duplex at bacterial replication fork requires hexameric helicase DnaB encircling lagging strand template and moving 5' to 3' direction using ATP hydrolysis energy. DnaB disrupts base pairing ahead of polymerase generating single-stranded templates for both leading and lagging synthesis simultaneously. Activity stimulated by DnaC loader during initiation and by tau subunit and SSB during elongation. DnaA protein recognizes oriC boxes but does not unwind long stretches, while DnaG synthesizes RNA primers after unwinding provides single-stranded regions.