Practice question

MCQ

Question

NATIVE-PAGE experiment for separation of proteins, if the amount of cross-linker is increased, what would happen?

Options

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Proteins will migrate to more distance No difference will be noted in distance to which protein will migrate because cross-linker only keep the gel hydrated Presence of more amount of cross-linker will lead to disruption of native structure of proteins There will be less separation among different proteins Correct

Explanation

Increasing bis-acrylamide cross-linker reduces gel pore size and may increase heterogeneity, restricting protein migration and diminishing resolution. Unlike SDS-PAGE, native PAGE retains conformation, so excessive crosslinking impedes separation rather than altering hydration or denaturing proteins.

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