Skip to content
Login Register

#SSR

3 public questions tagged with this topic.

Molecular Marker

Microsatellites are also known as:

Microsatellites consist of 1-6 base pair motifs tandemly repeated 5-50 times, dispersed throughout eukaryotic genomes. DNA polymerase slippage during replication creates extensive length polymorphism, making them multiallelic and highly informative. In molecular marker terminology, this class is designated as Simple Sequence Repeats (SSR) and Short Tandem Repeats (STR), whereas VNTR refers to minisatellites of 10-100 bp. SNPs involve single-base substitutions and ESTs are partial cDNA sequences. Thus microsatellite nomenclature aligns with STR, widely applied in forensic profiling and genetic mapping.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details
Molecular Marker

What is the main difference between SSR and SNP?

Simple sequence repeats and single nucleotide polymorphisms differ fundamentally in molecular basis and allelic diversity. SSR loci contain tandem repeats of short motifs where polymorphism arises from variation in repeat copy number, generating multiple alleles and high polymorphic information content. SNP represents a single base substitution, transition or transversion, at a specific genomic position, typically biallelic with lower information per locus but abundant genome-wide. SNP assays generally do not need radioactive labeling and differ in length scale from SSR amplicons.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details
Molecular Marker

Microsatellites are also called:

Microsatellites consist of short tandemly repeated DNA motifs of one to six base pairs, such as dinucleotide CA repeats, repeated five to fifty times. They are also termed simple sequence repeats because of this simple, repetitive organization. Their high mutation rate through DNA polymerase slippage during replication creates extensive length polymorphism. VNTR includes minisatellites with longer repeat units of ten to sixty base pairs, SNP denotes single base changes, and indels represent insertion-deletion polymorphisms, all structurally different from microsatellite architecture.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details