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#phospholipase C

3 public questions tagged with this topic.

Sea urchin -l

IP3 in fertilization is generated by:

Fertilization induces phosphoinositide signaling cascade generating crucial second messenger IP3. Phospholipase C isoforms, including sperm-contributed PLCζ and egg PLCγ activated by Src family tyrosine kinases downstream of sperm-receptor interaction, hydrolyze phosphatidylinositol 4,5-bisphosphate abundant in inner leaflet of plasma membrane. Cleavage yields membrane-retained diacylglycerol that activates protein kinase C and soluble inositol 1,4,5-trisphosphate that diffuses to endoplasmic reticulum. IP3 generation absolutely requires phospholipase C enzymatic catalysis; without PLC activity calcium wave fails. Protein kinase C, dynein ATPase and Na+/H+ exchanger act downstream modulating pH, cytoskeleton and motility but are not synthesizing IP3 upstream.

Ref: Carroll et al., Dev Biol 1997, Phospholipase C and IP3 generation at fertilization; Alberts Chapter 15.

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Cell Signaling GPCR Pathways - I

PLCβ hydrolyzes PIP2 to generate

IP3 and DAG, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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Cell Signaling GPCR Pathways - I

Gαq activates which effector enzyme?

Phospholipase Cβ, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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