Which enzyme is responsible for breaking peptide bonds in proteins?
Proteases (also called peptidases) break peptide bonds in proteins.
Ref: NCERT Class 11 Biology Chapter 9: Biomolecules Enzymes - Properties Classification and Cofactors
#peptide bonds
5 public questions tagged with this topic.
Filter questions
Which of the following biomolecules contains peptide bonds?
Proteins are made up of amino acids linked by peptide bonds.
Ref: NCERT Class 11 Biology Chapter 9: Biomolecules Chemical Analysis and Biomolecules - Amino Acids Lipids
What is the peak absorbance of peptide bonds in proteins?
Proteins display two principal UV absorption envelopes due to distinct chromophores. Aromatic residues phenylalanine, tyrosine, tryptophan absorb near 280 nm via π→π* transitions. Peptide backbone containing amide carbonyl undergoes separate transitions: intense π→π* around 190 nm and weaker n→π* shoulder near 210-220 nm. Maximum near 210 nm often used for peptide bond detection independent of aromatic content, valuable for peptides lacking tryptophan and tyrosine, quantifying number of peptide bonds. Absorbance at 210 nm complements A280, enabling total protein assessment and HPLC detection of eluting peptides with high sensitivity.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
Which of the following bonds primarily stabilizes the primary structure of proteins?
Peptide bonds accurately describes the structural composition or molecular organization asked about in this question. In Protein Structure, knowledge of molecular structure is directly linked to understanding biological function. The specific arrangement of chemical components in Peptide bonds determines its physical properties, biological activity, and interactions with other molecules. The other options (Hydrogen bonds, Disulfide bonds, and Ionic bonds) describe different structural arrangements, incorrect stoichiometry, or compositions of different biological molecules.
Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 4
Which of the following enzymes cleaves proteins at specific peptide bonds?
Trypsin is the scientifically accurate answer to this question. Within the study of Protein Degradation, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Trypsin directly address what is being asked. Among the other options, Peptidase, Ligase, and Kinase do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.
Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 4