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#micropropagation

6 public questions tagged with this topic.

Which of the following is correct about micropropagation?

Micropropagation is a tissue culture technique used to produce large numbers of genetically identical plants from a single tissue or ll. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Structural Organisation in Animals and Frog, Topic: Tissues, skeletal and organ systems.

Which plant tissue culture technique is used for large-scale plant propagation?

Micropropagation is used for large-scale production of genetically identical plants. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Zoology section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Zoology portion (Latest NCERT Textbooks for Academic Session 2026-27 - Rationalized Edition for Class XI and XII), Topic: Structural organization, physiology, human health and related concepts as per latest syllabus.

Shoot tip culture is an example of:

Shoot tip culture qualifies as organ culture because explant retains organized structure of shoot apical meristem plus one to three leaf primordia and continues organized growth maintaining polarity and tissue layer arrangement tunica corpus. Unlike callus culture where organization is lost into unorganized parenchyma, or cell suspension where aggregates disperse in liquid agitation, or protoplast culture where wall removed exposing naked membrane, shoot tip preserves defined zonation central zone peripheral zone rib meristem and procambium differentiation pathway. Culture on hormone balanced medium supports leaf expansion and internode elongation converting tip into miniature shoot that can be rooted. Since meristem remains intact, organ culture ensures high genetic stability avoiding mutation accumulation common in callus phase. Practical uses range from micropropagation of horticultural species to germplasm conservation under slow growth minimal medium and virus elimination when larger meristem is needed for survival in recalcitrant species where 0.1 mm meristem excision suffers low regeneration. Thus shoot tip culture bridges meristem culture and nodal culture methods for commercial clonal production.

Ref: George et al., Plant Propagation Tissue Culture Vol1; Hartmann organ culture.