Practice question
Question
Which of the following is not required for a PCR reaction?
Explanation
PCR requires Taq polymerase, deoxyribonucleoside triphosphates, primers and template DNA for amplification. Dideoxy-dNTPs lack 3'-OH and terminate chain elongation, used in Sanger sequencing, not PCR where continuous extension via normal dNTPs is essential.
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