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Question

CRISPR/Cas9 is an example of bacterial adaptive immunity. The transcription of CRISPR loci generates small crispr-RNAs (crRNA) to specifically target viral DNA, but not CRISPR loci, by forming complex with guide RNA and Cas9 nuclease. This prevention of autoimmunity is due to the:

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Explanation

nucleic acid structure and recombination analysis shows hairpin formation, Holliday junction resolution and translation control ensure fidelity. Consequently absence of protospacer adjacent motif sequence in CRISPR loci. emerges as the valid choice since it matches molecular mechanisms of gene expression, aligning with established principles in molecular biology literature.

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