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Question

A buffer contains 10% glucose, 20 mM Tris, and 50 mM HCI. For making 1 litre of buffer from the following stock solutions – 50% glucose, 1M Tris, and 1 M HCI, the correct combination of volume each of the stock solutions will be

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Explanation

enzyme catalysis and lipid structure analysis shows substrate affinity, transition state stabilization and phospholipid composition govern function. Consequently 200 ml, 20 ml, 50 ml emerges as the valid choice since it reflects catalytic efficiency and membrane organization, aligning with established principles in biochemistry literature.

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