Practice question

Question

You performed an experiment to create a gene knock out mice to study the function of gene X. During this experiment you prepared a plasmid containing a cloned gene X and two drug sensitivity genes neo and tk, which confer resistance to neomycin and sensitivity to ganciclovir, respectively. You then introduced the recombinant plasmid into the mouse ES cells. What it is purpose of including the neo and tk genes in the plasmid? (A) Gene tk is used to make cells with non-homologous recombination ganciclovir sensitive. (B) Gene neo is used to select transfected cells. (C) Gene neo is used to make cells with non-homologous recombination neomycin sensitive. (D) Gene tk is used to select transfected cells. Choose the correct answer from the options given below :