Practice question
Question
Which of the chemicals are used in the preparation of metaphase chromosomes for analysis of light microscopy? (A) Phytohemagglutinin (B) Quinacrine (C) Colchicine (D) Hypotonic saline Choose the correct answer from the options given below:
Explanation
Phytohemagglutinin stimulates T-lymphocyte mitosis in blood cultures. Colchicine arrests cells at metaphase by depolymerizing microtubules. Hypotonic saline swells cells for chromosome spreading. Quinacrine is fluorochrome for Q-banding, not metaphase preparation per se, hence A, C, D combination is used.
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