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Question

What is the mode of action of exonuclease III?

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Explanation

Exonuclease III predominantly digests double-stranded DNA from blunt ends or 3'-recessed termini. It hydrolyzes phosphodiester bonds in the 3' to 5' direction releasing 5'-mononucleotides, while lacking activity on single-stranded DNA or 3' overhangs longer than four bases, useful for deletion cloning.

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