Practice question
Question
The polymerization of the gel used in PAGE occurs between polyacrylamide and:
Explanation
Polyacrylamide gel for electrophoresis forms by polymerization of acrylamide monomers crosslinked with N,N'-methylene-bis-acrylamide initiated by APS and TEMED. Bis-acrylamide creates matrix pores whose size determines protein separation. Other named acrylamides are incorrect chemical identifiers for crosslinker.
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