Practice question
Question
In gel electrophoresis, which of the following molecule will move faster if the amount of DNA present is same in all?
Explanation
Supercoiled plasmid DNA is most compact conformation with reduced hydrodynamic radius and greater charge density, migrating fastest through agarose matrix under electric field. Nicked open-circular form is bulkiest and slowest, while linear migrates intermediate when identical mass loads.
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