Practice question
Question
Hybridomas are immortalized for production of unlimited supply of monoclonal antibodies by which of the following:
Explanation
Hybridoma technology fuses antibody-producing B lymphocytes with immortal myeloma cells using polyethylene glycol or electrofusion. Resulting hybrid cells retain B-cell immunoglobulin secretion and myeloma immortality, selected in HAT medium, providing continuous unlimited monoclonal antibody production for research and therapy.
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