Practice question
Question
Competent cells prepared in your lab have a transformation efficiency of 10 ⁸ cfu/μg of plasmid DNA. These competent cells (100 μl) were transformed with 10 ng of plasmid DNA using heat shock method followed by addition of 900 μl of LB medium. 100 μl of transformed cells were plated on antibiotic containing plates. The number of colonies expected to be on the plate would be:
Explanation
The principle of nucleic acid structure and recombination explains that hairpin formation, Holliday junction resolution and translation control ensure fidelity. Hence 10 ² fits best because it matches molecular mechanisms of gene expression, as documented in authoritative sources on molecular biology.
Discussion
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