Practice question

MCQ

Question

Cell cycle regulatory genes (cdc) were originally discovered by Paul Nurse in yeasts using genetic approach involving temperature sensitive mutant screening. Which of the following was used to identify cdc?

Options

Choose one · Correct answer highlighted

Genome sequencing cDNA complementation assay Correct RNAi method Homologous recombination

Explanation

The principle of cellular compartmentalization and cell cycle explains that SNARE-mediated trafficking, Hayflick limit, P53 and cyclin regulation control proliferation. Hence cDNA complementation assay fits best because it aligns with membrane trafficking and cell cycle control, as documented in authoritative sources on cell biology.

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