Practice question
Question
An enzyme of 40 kDa is added to a substrate solution in a molar ratio of 1:3. The concentration of the enzyme in the mixture is 12 mg/ml. What would be the corresponding substrate concentration?
Explanation
The principle of enzyme kinetics explains that substrate binding and catalytic efficiency determine reaction rates. Hence 0.9 mM fits best because it reflects active-site specificity and regulation, as documented in authoritative sources on biochemistry.
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