Practice question
Question
A recombinant protein is expressed in E. coli under T7 promoter at 37 ᵒ C. However no biological activity is obtained in the cell lysate. If the same experiment is carried out at 25 ᵒ C, the cell lysate shows a reasonable biological activity. The most probable explanation for this is:
Explanation
Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates recombinant protein is properly folded at low temperature because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.
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