Practice question
Question
A PCR was setup to amplify a 500 nucleotides-long DNA. The dNTPs in the reaction mixture were radiolabeled. The percentage (%) of radiolabeled singlestranded DNA after three cycles will be ______ (up to one decimal place) (NAT)
Explanation
The principle of DNA replication fidelity explains that polymerase proofreading and origin firing ensure accurate duplication. Hence Option NAT fits best because it matches high-fidelity synthesis mechanisms, as documented in authoritative sources on molecular biology.
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