Practice question
Question
A PCR in a 100 Micro L reaction volume, containing two primers at a concentration of 0.2 Micro M each, is set up to amplify a 250 base pair DNA fragment. Consider the average molecular weight of one base pair as 660 Da. If the primers are fully consumed by the end of the reaction, the amount of the final PCR product formed is Micro g (rounded off to one decimal place).
Explanation
The principle of DNA replication fidelity explains that polymerase proofreading and origin firing ensure accurate duplication. Hence Option NAT fits best because it matches high-fidelity synthesis mechanisms, as documented in authoritative sources on molecular biology.
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