Practice question
Question
A DNA fragment was cloned at the EcoRI restriction site of a plasmid vector that contains antibiotic resistance genes, kanR and specR. It was observed that all the positive clones (containing the DNA fragment of interest) grew on medium with kanamycin but not on media containing spectinomycin. In the absence of any other confounding factors, which one of the following statements would explain this observation?
Explanation
nucleic acid structure and recombination analysis shows hairpin formation, Holliday junction resolution and translation control ensure fidelity. Consequently The EcoRI site was located within the spec ᴿ gene. emerges as the valid choice since it matches molecular mechanisms, aligning with established principles in molecular biology literature.
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