Practice question

Question

A continuous cell culture being carried out in a stirred tank reactor is described in terms of its cell mass concentration X and substrate concentration S. The concentration of the substrate in the sterile feed stream is SF = 10 g/L and yield coefficient Yx/s = 0.5. The flow rates of the feed stream and the exit stream are equal (F=5 mL/min) and constant. If the specific growth rate (h ⁻¹ ) 𝜇 = 0.3 𝑆/(1+𝑆), the steady state concentration of S is _____ g/L (up to 1 decimal point).