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[Co(en)3]3+ (octahedral) with 3 bidentate en ligands lacks symmetry, forming non-superimposable mirror images (enantiomers).
Ref: NCERT Class 12 Chemistry > Chapter 5: Coordination Compounds > Topic: Isomerism - Geometrical Isomerism in Coordination Compounds
Enantiomers are non-superimposable mirror images with identical mass, ultraviolet absorption and hydrophobic properties but opposite chiroptical interaction. Left and right circularly polarized light absorption differs in sign, so CD spectrum of one enantiomer is mirror image of the other, with inverted ellipticity. This property allows determination of absolute configuration and enantiomeric excess in drug development, carbohydrate and amino acid analysis. Mass spectrometry cannot distinguish enantiomers without chiral additive, ultraviolet shift senses conjugation not chirality. CD spectra difference provides decisive chiral discrimination.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
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