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#PCR

72 public questions tagged with this topic.

Which component is essential for initiating the Polymerase Chain Reaction (PCR)?

Primers are short sequences of nucleotides essential for initiating DNA synthesis in PCR. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.

Which of the following is an advantage of PCR over traditional cloning methods?

PCR is a rapid method that can amplify DNA sequences within hours, unlike traditional cloning, which takes days or weeks. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Zoology section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Zoology portion (Latest NCERT Textbooks for Academic Session 2026-27 - Rationalized Edition for Class XI and XII), Topic: Structural organization, physiology, human health and related concepts as per latest syllabus.

Which of the following statements about polymerase chain reaction (PCR) is incorrect?

PCR involves temperature cycling, including denaturation, annealing, and extension steps. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Zoology section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Zoology portion (Latest NCERT Textbooks for Academic Session 2026-27 - Rationalized Edition for Class XI and XII), Topic: Structural organization, physiology, human health and related concepts as per latest syllabus.

Which of the following statements about polymerase chain reaction (PCR) is correct?

PCR is a technique used to amplify specific DNA sequences using thermostable enzymes like Taq polymerase and primers. This follows from NCERT principle where relation explains outcome clearly for students.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Biology - Zoology portion covering relevant system and function, Topic: Structural organization and physiology.

Which of the following statements about PCR is incorrect?

PCR involves a series of temperature cycles, including denaturation, annealing, and extension. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Biology - Zoology portion covering relevant system and function.

Which of the following is correct about polymerase chain reaction (PCR)?

PCR is a technique used to amplify specific DNA sequences through cycles of denaturation, annealing, and extension. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.

Which of the following is a key advantage of PCR over traditional cloning methods?

PCR is a rapid method for amplifying DNA sequences within hours, unlike traditional cloning, which takes days or weeks. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.

Which method is used to amplify a specific DNA segment?

Polymerase Chain Reaction (PCR) is used to amplify specific DNA segments in vitro. This follows from NCERT principle where relation explains outcome clearly for students.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Biology - Zoology portion covering relevant system and function, Topic: Structural organization and physiology.

Why are thermostable enzymes used in PCR?

Thermostable enzymes, such as Taq polymerase, are used in PCR because they remain active during high-temperature DNA denaturation. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Zoology section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Zoology portion (Latest NCERT Textbooks for Academic Session 2026-27 - Rationalized Edition for Class XI and XII), Topic: Structural organization, physiology, human health and related concepts as per latest syllabus.

Why are thermostable enzymes used in PCR?

Thermostable enzymes, such as Taq polymerase, are used in PCR because they remain active during high-temperature DNA denaturation. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.