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#molecular weight

7 public questions tagged with this topic.

What is the molecular weight of the Arp2/3 complex?

Arp2/3 complex purification and characterization resolved molecular composition and mass. Original isolation from Acanthamoeba by Pollard demonstrated seven polypeptides co fractionating through multiple chromatographic steps with actin binding activity. Subunit sequencing identified two actin related proteins Arp2 44 kilodalton and Arp3 50 kilodalton sharing actin fold, plus ARPC1 p40 WD repeat beta propeller 40 kilodalton, ARPC2 p34 34 kilodalton, ARPC3 p21 21 kilodalton, ARPC4 p20 20 kilodalton, ARPC5 p16 14 to 16 kilodalton. Sum calculated 224 kilodalton, sucrose gradient sedimentation and gel filtration elution volume around 200 to 220 kilodalton confirming monomeric assembly. Cryo electron microscopy volume consistent with 15 nanometer globular particle. Higher mass 400 to 600 kilodalton would imply dimer or association with nucleation promoting factor, lower 30 to 50 corresponds to actin monomer, 100 to 150 would be incomplete subcomplex lacking several subunits, insufficient for nucleation. Therefore assignment 200 to 220 kilodalton aligns with structural and biochemical consensus for intact active complex and functional unit.

Ref: Pollard and Earnshaw, Cell Biology, Chapter 12: Arp2/3 Complex Molecular Weight and Composition.

What is the primary factor responsible for DNA separation in electrophoresis?

In electrophoresis, migration depends on charge to mass ratio and molecular sieving. DNA possesses a uniform negative charge due to phosphate backbone, one per nucleotide, resulting in nearly identical charge/mass ratio for all fragments. Consequently, in free solution, all DNA migrates equally. Separation in agarose or polyacrylamide occurs due to frictional resistance of the porous matrix, where longer molecules experience greater retardation. Thus, although labeled as size-based separation, the underlying principle involves differential mobility governed by charge/mass ratio interacting with sieving and frictional forces.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

What will be the molarity of 1 mg/mL protein solution if its molecular weight is 10 kDa?

0.1mM is the scientifically accurate answer to this question. Within the study of Water and Mole Concept, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of 0.1mM directly address what is being asked. Among the other options, 1mM, 10mM, and 100mM do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

Which amino acid has the highest molecular weight among the standard amino acids?

Tryptophan is the scientifically accurate answer to this question. Within the study of Amino Acids Basics, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Tryptophan directly address what is being asked. Among the other options, Arginine, Leucine, and Phenylalanine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 3

Which amino acid has the lowest molecular weight?

Glycine is the scientifically accurate answer to this question. Within the study of Amino_Acids_Structure, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Glycine directly address what is being asked. Among the other options, Alanine, Serine, and Cysteine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Campbell Biology, Urry et al., 12th Ed.

Which amino acid has the highest molecular weight?

Tyrosine is the scientifically accurate answer to this question. Within the study of Amino_Acids_Structure, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Tyrosine directly address what is being asked. Among the other options, Tryptophan, Methionine, and Arginine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Campbell Biology, Urry et al., 12th Ed.