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#gynogenesis

2 public questions tagged with this topic.

Plant tissue culture 2

Gynogenesis differs from androgenesis because it uses:

Gynogenesis differs from androgenesis because it uses female gametophyte components unfertilized ovules ovaries or embryo sacs as explant source rather than anther pollen. In gynogenesis egg cell synergid or antipodal cells within embryo sac are induced to develop sporophytically into haploid embryo without fertilization, typically by culturing unpollinated ovaries or ovules on sucrose enriched medium with auxin cytokinin combination. Female gametophyte origin ensures captured genome is that of maternal parent, contrasting with androgenesis capturing paternal genome from microspores. Gynogenes

Ref: Yang & Zhou Theor Appl Genet gynogenesis; Bohanec 2009 haploid production review.

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Plant Breeding 4

Gynogenesis involves culture of:

Gynogenesis derives haploid plants from female gametic lineage, typically through in vitro culture of unpollinated ovules, ovaries, or isolated embryo sacs under defined media conditions. Under specific culture conditions, cells of embryo sac such as egg cell, synergids, antipodals, or nucellar tissue undergo sporophytic development without fertilization, forming haploid embryo with maternal genome and maternal cytoplasm. Unlike androgenesis that samples male gametes, gynogenesis recovers maternal genotype useful when male gametophyte is recalcitrant or expresses self-incompatibility leading t

Ref: Hosoki T & Asahira. Gynogenesis – ovule culture; Bohanec B 2009 – review of gynogenic haploid breeding

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