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#gene mutations

4 public questions tagged with this topic.

Complementation analysis is used to determine whether

Complementation analysis distinguishes whether recessive mutations causing same phenotype affect same functional unit or different transcriptional units. Two homozygous mutants crossed or heterokaryon formed produce trans heterozygote carrying one mutant allele from each parent on homologous chromosomes. If each genome supplies wild-type product compensating other's defect, wild-type phenotype restored indicating mutations complement and reside in different genes. Failure to restore indicates both lesions disrupt same cistron with no intact copy available, grouping mutants into single complementation group representing one gene.

Ref: Hartl & Ruvolo, Genetics, 9th ed., Chapter 7: Complementation Test for Functional Alleles and Cistrons

AP3/PI mutants show replacement of petals and stamens by:

Go with A — Sepals and carpels. Under ABC MODEL, this is the standard explanation you’d use in class: it names the real driver or definition, while the rest are nearby but wrong. Not these: B) Carpels only; C) Sepals only; D) Petals only. When two options sound similar, choose the one that matches the textbook definition most tightly.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.

Which whorls are affected in agamous (AG) mutants?

That points to C: Stamens and carpels. Compared with the other options, Stamens and carpels is the one that correctly describes the ABC MODEL concept. The wrong ones are A) Sepals and petals; B) Petals and stamens; D) Sepals and carpels. If you’re stuck, eliminate anything that contradicts a basic fact you already know for this topic.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.